Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440
نویسندگان
چکیده
منابع مشابه
Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440
BACKGROUND Pseudomonas putida KT2440 is able to synthesize large amounts of medium-chain-length polyhydroxyalkanoates (mcl-PHAs). To reduce the substrate cost, which represents nearly 50% of the total PHA production cost, xylose, a hemicellulose derivate, was tested as the growth carbon source in an engineered P. putida KT2440 strain. RESULTS The genes encoding xylose isomerase (XylA) and xyl...
متن کاملAmino acid racemization in Pseudomonas putida KT2440.
D-Amino acids have been shown to play an increasingly diverse role in bacterial physiology, yet much remains to be learned about their synthesis and catabolism. Here we used the model soil- and rhizosphere-dwelling organism Pseudomonas putida KT2440 to elaborate on the genomics and enzymology of d-amino acid metabolism. P. putida KT2440 catabolized the d-stereoisomers of lysine, phenylalanine, ...
متن کاملPRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM PALMITIC ACID USING Ralstonia eutropha BY FED BATCH CULTURE
Polyhydroxyalkanoates (PHA’s) are carbon and energy storage compounds which accumulate intracellulary as granules in certain bacteria during unbalanced growth and in the presence of an excess carbon source. PHAs were well studied regarding their promising applications, physical, chemical and biological properties. PHAs are biodegradable, biocompatible, have good material properties, renewable a...
متن کاملProduction of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida
BACKGROUND Pseudomnas putida is a natural producer of medium chain length polyhydroxyalkanoates (mcl-PHA), a polymeric precursor of bioplastics. A two-fold increase of mcl-PHA production via inactivation of the glucose dehydrogenase gene gcd, limiting the metabolic flux towards side products like gluconate was achieved before. Here, we investigated the overproduction of enzymes catalyzing limit...
متن کاملProduction of selenium nanoparticles in Pseudomonas putida KT2440
Selenium (Se) is an essential element for the cell that has multiple applications in medicine and technology; microorganisms play an important role in Se transformations in the environment. Here we report the previously unidentified ability of the soil bacterium Pseudomonas putida KT2440 to synthesize nanoparticles of elemental selenium (nano-Se) from selenite. Our results show that P. putida i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2012
ISSN: 1472-6750
DOI: 10.1186/1472-6750-12-53